An arginine-functionalized stationary phase for hydrophilic interaction liquid chromatography.
نویسندگان
چکیده
An arginine-functionalized stationary phase for hydrophilic interaction liquid chromatography (HILIC) has been prepared for the first time by clicking arginine onto silica gel. It offers an efficient separation of organic acids, nucleotides and sugars. More interestingly, it exhibited excellent selectivity and enrichment toward acidic glycopeptides, even at a ratio of 1 : 150 to non-glycopeptides.
منابع مشابه
Performance of 2-Amino Tetraphenyl Porphyrin as Stationary Phase in RP-HPLC of Amino Acids
The search for new stationary phases has been one of the predominant concerns in high performance liquid chromatography (HPLC) in order to achieve better resolutions, longer column lives, and reduce the time of analysis. A chromatographic packing for separation of underivatized amino acids (AAs) were prepared by dynamically coating 2-amino tetraphenyl prophyrin (atpp) on a C-18 reversed-pha...
متن کاملHydrophilic interaction liquid chromatography (HILIC)—a powerful separation technique
Hydrophilic interaction liquid chromatography (HILIC) provides an alternative approach to effectively separate small polar compounds on polar stationary phases. The purpose of this work was to review the options for the characterization of HILIC stationary phases and their applications for separations of polar compounds in complex matrices. The characteristics of the hydrophilic stationary phas...
متن کاملA polyvinyl alcohol-coated silica gel stationary phase for hydrophilic interaction chromatography.
Multiple layers of polyvinyl alcohol (PVA) coating are generated onto silica gel by thermal immobilization to form a stationary phase applied for hydrophilic interaction liquid chromatography (HILIC). It offers an easy way to manipulate the thickness of PVA coating and the obtained stationary phase demonstrated high efficiency and high chemical stability.
متن کاملPolyhydroxyl and Polyphosphorylcholine Functionalized Silica for Hydrophilic Interaction Liquid Chromatography Synthesis, Characterization and Application
This thesis focuses on the development of new stationary phases for use in hydrophilic interaction liquid chromatography using TRIS‐based and phosphorylcholine typed monomers and porous silica particles as starting substrates. In this thesis, several ways of polymerizing highly hydrophilic mono‐ mers onto pore surfaces of silica supports are described, based on several “grafting from” schemes. ...
متن کاملPreparation and characterization of surfactin-modified silica stationary phase for reversed-phase and hydrophilic interaction liquid chromatography.
Surfactants are good candidates as selectors in mixed-mode reversed-phase liquid chromatography (RPLC)/hydrophilic interaction liquid chromatography (HILIC) because they contain both a hydrophobic and a hydrophilic moiety. Surfactin, a cyclic heptapeptide, is an efficient biosurfactant produced by Bacillus subtilis that comprises seven amino acids and a β-hydroxyl fatty acid. A surfactin-modifi...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- The Analyst
دوره 140 12 شماره
صفحات -
تاریخ انتشار 2015